Lysine Separtion by Ion Exchange using Amberlite IR-20

Author : Tan, Mary Jane P.
Major Adviser : Chay, Pham Binh
Committee Members :
Year : 1990
Month : March
Type : Thesis
Degree: BS
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Production of L-lysine has received increased attention over the past years. However, the conditions necessary for lysine separation and recovery from the fermentation broth still need further investigation. This experiment evaluated the adsorption-desorption process using Amberlite IR-120. Two methods were used which are the static or batch test, to determine the optimum conditions for adsorption; and the column adsorption-desorption to determine the effect of feed rate and weight of resin in the adsorption process, and the effect of eluent concentration in the desorption process.

Maximum adsorption was obtained at pH 8, at the level where lysine has two net positive charges and which is also below the isoelectric pH (IEP) value. However, temperature had no significant effect on the adsorption of lysine.

Adsorptive capacity of the resin increased with increase in concentration. Adsorption at least doubled when the weight of resin increased from 5 to 10 g. Lower volume of the effluent was used to reach the saturation point of the resin at a slower feed rate. At this point, maximum adsorption was effected.

On the other hand, almost complete desorption (~98%) was obtained using smaller volume of eluent with higher concentration. However, eluent volume greatly increased as the amount of resin to be eluted was increased. Significant results on the 30-fold increase of lysine obtained in the acid mixture.

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