Parametric Study on Acetone-Butanol-Ethanol (ABE) Fermentation of Sweet Sorghum (Sorghum bicolor ‘SPV 422′) Syrup using Clostridium acetobutylicum 1532

Author : Abasula, Leah Jane Ponayo
Major Adviser : Demafelis, Rex B.
Committee Members : Movillon, Jovita A.; Gatdula, Kristel M.; Bambase Jr., Manolito E.; Dizon, Lisa Stephanie H.
Year : 2017
Month : June
Type : Thesis
Degree: BS
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ABE fermentation of sweet sorghum syrup was performed using Clostridium acetobutylicum 1532. The effects of different parameters on the fermentation efficiency were determined. The parameters used were temperature (30 °C and 50 °C), Brix (10 °Brix and 30 °Brix), inoculum loading (10% and 30%), pH (4 and 6), and nutrient supplementation (with and without ammonium acetate). Comparative run was done in order to determine the effect of agitation. Two-level fractional factorial design (2ᵏ⁻¹) was used, generating 16 runs, and this was done in duplicate. For the parametric run, agitation was not used as parameter. Run 12 produced the highest butanol concentration with 0.049 %v/v. The parameters for run 12 are 50 °C, 30 °Brix, 10% inoculum loading, pH 6 and without nutrient supplementation. While run 9 has the highest fermentation efficiency at 2.84%. The parameters for run 9 are 30 °C, 10 °Brix, 10% inoculum loading, pH 6 and without nutrient supplementation. Both pH and interaction of substrate concentration and nutrient supplementation have negative significance on fermentation efficiency. The fermentation condition for the comparative analysis was set at parameters of run 12 since it has the highest butanol production from the parametric runs. The effect of agitation was determined in the comparative run. It was found out that fermentation set-up with agitation has higher butanol concentration (0.068 %v/v) compared to without agitation (0.061 %v/v).

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