Parametric Study on the Acetone-Butanol-Ethanol (ABE) Fermentation of Sugarcane (Saccharum officinarum, L.) Molasses using Clostridium acetobutylicum 1200

Author : Garcia, Christian Santiago
Major Adviser : Demafelis, Rex B.
Committee Members : Bambase Jr., Manolito E.; Dizon, Lisa Stephanie H.; Gatdula, Kristel M.; Movillon, Jovita A. 
Year : 2017
Month : June
Type : Thesis
Degree: BS
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Acetone-Butanol-Ethanol fermentation of sugarcane molasses has been performed using Clostridium acetobutylicum 1200 considering different parameters such as pH level (4 and 6), substrate concentration (10 °Brix and 30 °Brix), inoculum loading (10% v/v and 30% v/v), nutrient supplementation (with and without), fermentation temperature (30 °C and 50 °C) and agitation (with and without). Sixteen samples with duplicates underwent fermentation for 72 hours and are subjected to gas chromatography and Miller’s DNS method to quantify the fermentation efficiency of set-ups using Design Expert v.10.0.0. Temperature, substrate concentration, the interaction between pH and nutrient supplementation, and the interaction between temperature and pH were the parameters found to be of significant effect. Decreasing temperature and substrate concentration has a positive effect on both the fermentation efficiency and butanol production of the set-ups. 30 °C, pH 4, 30 °Brix, 10% v/v, without nutrient supplementation gave off the highest butanol concentration (0.0200% v/v) and total solvents concentration during the 16 runs duplicates while set-up on 30 °C, pH 4, 10 °Brix, 10% v/v, with nutrient supplementation gave the highest fermentation efficiency of 3.1140 %. Furthermore, the effect of agitation was proven to be insignificant.

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