Kinetic Analysis of Lysine Production for Different Process Conditions of Biotin, Homoserine and Oxygen-enriched Air

Author : Opulencia, Timotea P.
Major Adviser : Chay, Pham Binh
Committee Members : Acda, Reynaldo I.; Movillon, Jovita A. 
Year : 1993
Month : April
Type : Thesis
Degree: BS
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A study on the effect of biotin, homoserine and oxygen-enriched air on the kinetics of batch lysine production was conducted using a homoserine auxotroph Corynebacterium glutamicum 9NG7.

Varying concentration of biotin and homoserine done using shake-flask cultures showed an increased. Low yields were observed for this method in contrast to what has been attained on the experiment done on a stirred-tank fermentor which was used for the study on the effect of oxygen-enriched air. Increasing the level of dissolved oxygen increased also the yield with the level controlled on a certain range and not on a fixed value. The highest lysine concentration obtained was 43 g/l with 31.5 percent conversion.

Through analysis of variance it was found out that shake flasks data have a significant differences  on lysine yield for varying biotin concentrations while varying the concentration of homoserine showed no significant differences on its effect on product formation.

Interaction effects were studied and multiple regression analyses were applied which gave the predicting equations for the three dependent variables. The equations that have been developed were used to predict  the critical values of lysine and biomass yield and the residual sugar concentration. From the analysis, a high positive correlation was determined between the dependent and independent variables.

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