Parametric Study on Acetone-Butanol-Ethanol (ABE) Fermentation of Sugarcane (Saccharum officinarum, L.) Molasses using Clostridium acetobutylicum 1532

Author : Mancenido, Jeremiah Lawrence Reanoga
Major Adviser : Demafelis, Rex B.
Committee Members : Bambase Jr., Manolito E.; Dizon, Lisa Stephanie H.; Gatdula, Kristel M. 
Year : 2017
Month : June
Type : Thesis
Degree: BS
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The effects of different parameters of temperature, sugar concentration, inoculum loading, pH, nutrient supplementation and their interactions were assessed from the fermentation efficiency based on the butanol concentration produced and the reducing sugar consumed by Clostridium acetobutylicum 1532 in the conversion of molasses to alcohol. The samples were analyzed at time 0 and 72 hours. Miller’s DNS analysis was employed for the absorbance read using a UV-VIS Spectrophotometer while the determination of the solvent concentration was conducted using Gas Chromatography. The significant factors considered after statistical analyses were initial pH, °Brix, interaction of the initial pH and inoculum loading and the interaction of the initial pH with temperature. The highest observed fermentation efficiency, on the average, was 1.74%. The comparative run was done for the time profiling every eight hours for butanol formation and the corresponding reducing sugar consumption with the presence and absence of agitation. The highest values of the volume fraction of butanol produced in runs with and without agitation are 0.0545% and 0.0565%, respectively. It was concluded that there was no observable effect of agitation for butanol formation while an increased consumption of the reducing sugar content. From the ANOVA summary, it was concluded that for this microorganism, the effect of agitation is insignificant.

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