Parametric Study on Acetone-Butanol-Ethanol (ABE) Fermentation of Sugarcane Syrup using Clostridium acetobutylicum 1200

Author : Custodio, Janin Francisco
Major Adviser : Demafelis, Rex B.
Committee Members : Bambase Jr., Manolito E.; Movillon, Jovita A.; Dizon, Lisa Stephanie H.; Gatdula, Kristel M. 
Year : 2017
Month : June
Type : Thesis
Degree: BS
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Acetone-Butanol-Ethanol (ABE) fermentation of sugarcane syrup using Clostridium acetobutylicum 1200 was conducted to investigate the effect of incubation temperature (30 °C , 50 °C), substrate concentration (10 °Bx, 30 °Bx), inoculum loading (10 %v/v, 30 %v/v), pH (pH 4, pH 6), and medium nutrient supplementation (without, 0.6 g/100 mL). In this work, it was established that pH, temperature, substrate concentration, and nutrient supplementation significantly affects the fermentation efficiency (based on butanol production) of C. acetobutylicum 1200 using the said feedstock. Maximum butanol concentration of 0.071 %v/v was recorded at 30 °C, pH 6, 30 °Bx, 10 %v/v inoculum loading, and with nutrient supplementation. Maximum fermentation efficiency, equal to 5.14%, was obtained at 30°C, 10 °Bx, 30 %v/v, pH 6, and with nutrient supplementation. Among the four significant factors, only substrate concentration was found to be different from the run that resulted to maximum butanol production, this was due to the lower value of initial fermentable sugars of the former than the latter that favored higher efficiency. In addition, a comparative study was conducted on the parametric setup with highest butanol yield for the assessment of agitation effect and for the construction of butanol-sugar concentration profiles. For both conditions, butanol concentration increased while reducing sugar concentration decreased with time. Presence of agitation insignificantly increased the fermentation efficiency from 3.39% to 3.59%.

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